Green Qpcr Primers

The azuraquant green fast qpcr mix is a ready to use 2x master mix for use in real time quantitative pcr assays in which intercalating dye based detection provides the option of a post amplification melt profile.

Green qpcr primers.

General description overview compatible with any thermal cycler kicqstart primers are ready to order pre designed primer pairs for quantifying gene expression by sybr green i rt qpcr reverse transcription quantitative real time pcr. The primers are meticulously designed using origene s proprietary primer design algorithm developed from 10 000 qpcr experiments. The mircury lna mirna pcr assays and panels have been validated using the mircury sybr green master mix. The kit also includes a control primer set designed for amplification of the synthetic rna spike in provided in the mircury lna rt kit.

Use of other reagents for qpcr will affect the results. Sybr green for qpcr. Qstar qpcr primer pairs are designed for sybr green based real time qpcr. Mark livingstone image 1 design qpcr primers image1 jpg image 2 design qpcr primers image2 page 1 jpg image 3 design qpcr primers image2 page 2 jpg.

Refer to the mircury lna rt kit handbook for details. Our primer collection covers the entire human and mouse genomes. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. To comply with miqe they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off target amplification 1.

Design sybr green qpcr primers experimental protocol oct 31 2013 recommendations.